"Micro- and Nano- Transport of Biomolecules" by David Bakewell
BOBOCOAE, DB&VENTUS PUBLISHING APS | 2009 | ISBN: 8776815134 9788776815134 | 96 PAGES | PDF |5.36 MB
This book introduces to biomolecules and describes the experimental and theoretical aspects of their micro- and nano-scale motion in water. Particular emphasis is given to their transport in engineered micro-environments where they are driven by externally imposed electric fields. Envisaged application technologies of this wide-ranging science involve healthcare, food provisioning, environmental services, etc. The e-book is generally intended for undergraduate students studying chemical, life, physical and engineering sciences, and also interdisciplinary researchers.Contents
Preface
1 Introduction
1.1 Motivation: biomolecules in scientifi c context
1.2 Length scale of transport
1.3 Biomolecule transport example: engineered microdevices
1.4 Structure of this e-book
2 Biomolecules and their electrical properties
2.1 Biomolecules in cells
2.2 Biomolecules: structure and function
2.2.1 Nucleic acids
2.2.2 Proteins
2.2.3 Carbohydrates
2.2.4 Lipids
2.3 Biomolecules: electrical properties
2.3.1 Polyelectrolytes
2.3.2 DNA can be modeled as wormlike chain
2.3.3 Biomolecules and bioparticles
2.3.4 Electrical double layer
2.3.5 Introduction to dielectric polarization
2.3.6 Polarisation parameters: a brief view
2.3.7 Measurement of biomolecule polarisation parameters
2.4 Concluding remarks
3 Moving biomolecules using electric fi elds
3.1 Electrophoresis
3.2 Dielectrophoresis (DEP)
3.2.1 Polarisation and DEP biomolecule transport
3.2.2 Maxwell-Wagner interfacial polarisation
3.2.3 Maxwell-Wagner interfacial polarisation for bioparticles
3.2.4 Maxwell-Wagner polarisation for DNA
3.2.5 Counterion fl uctuation polarisation
3.2.6 Counterion fl uctuation polarisation for bioparticles
3.2.7 Counterion fl uctuation polarisation for DNA
3.2.8 Other polarisation mechanisms
3.3 Micro-environments for biomolecule transport
3.4 Concluding remarks
4 Basic micro- and nano-transport
4.1 Inertial, friction and sedimentation forces on single biomolecules
4.2 Electromagnetic forces acting on single biomolecules
4.2.1 Electric fi elds and electrophoresis
4.2.2 Inhomogenous electric fi elds and dielectrophoresis
4.2.3 Electroosmosis
4.2.4 Magnetic fi elds
4.3 Thermal fl uctuations
4.4 Combining forces for predicting single bioparticle trajectory
4.5 Langevin equation for a single bioparticle (biomolecule)
4.6 Langevin equation stochastic integration and the modifi ed diffusion equation (MDE)
4.6.1 Example of one-dimensional (1D) MDE transport
4.6.2 1D MDE transport parameters
4.6.3 3D MDE transport and parameters
4.7 Concluding remarks
5 Observing, quantifying and simulating electrically driven biomolecule microtransport
5.1 Micro-device and experimental arrangement
5.2 Observations and quantitative measurements
5.2.1 Using geometry of DEP force aids quantifi cation
5.2.2 DEP collections exhibit frequency and voltage dependence
5.3 Simulations of electrically driven biomolecule micro-transport
5.3.1 Determining the dielectrophoretic force throughout the chamber.
5.3.2 Solutions of the MDE for predicting bioparticle collections
5.4 Brief discussion of experiments and theory
5.5 Concluding remarks
6 References
6.1 General – selected books
6.2 Research articles and other reading
with TOC BookMarkLinks
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